Title | HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Fries CN, Chen J-L, Dennis ML, Votaw NL, Eudailey J, Watts BE, Hainline KM, Cain DW, Barfield R, Chan C, M Moody A, Haynes BF, Saunders KO, Permar SR, Fouda GG, Collier JH |
Journal | Sci Rep |
Volume | 11 |
Issue | 1 |
Pagination | 14494 |
Date Published | 2021 Jul 14 |
ISSN | 2045-2322 |
Keywords | Animals, Female, Germinal Center, Herpes Simplex Virus Vaccines, HIV Antibodies, HIV Antigens, HIV Envelope Protein gp120, Immunoglobulin G, Mice, Inbred C57BL, Nanofibers, T-Lymphocytes, Helper-Inducer |
Abstract | A major challenge in developing an effective vaccine against HIV-1 is the genetic diversity of its viral envelope. Because of the broad range of sequences exhibited by HIV-1 strains, protective antibodies must be able to bind and neutralize a widely mutated viral envelope protein. No vaccine has yet been designed which induces broadly neutralizing or protective immune responses against HIV in humans. Nanomaterial-based vaccines have shown the ability to generate antibody and cellular immune responses of increased breadth and neutralization potency. Thus, we have developed supramolecular nanofiber-based immunogens bearing the HIV gp120 envelope glycoprotein. These immunogens generated antibody responses that had increased magnitude and binding breadth compared to soluble gp120. By varying gp120 density on nanofibers, we determined that increased antigen valency was associated with increased antibody magnitude and germinal center responses. This study presents a proof-of-concept for a nanofiber vaccine platform generating broad, high binding antibody responses against the HIV-1 envelope glycoprotein. |
DOI | 10.1038/s41598-021-93702-x |
Alternate Journal | Sci Rep |
PubMed ID | 34262096 |
PubMed Central ID | PMC8280189 |
Grant List | R01 AI145016 / AI / NIAID NIH HHS / United States 1R01AI145016 / NH / NIH HHS / United States T32GM008555 / NH / NIH HHS / United States UL1TR002553 / TR / NCATS NIH HHS / United States |